Centrifugal pellet with acetone, ether, washed repeatedly skim to get the crude polysaccharides, papain addition to the protein in the crude polysaccharides, activated carbon removal of pigment, using the phenol sulfuric acid method for the determination of the polysaccharide content

After the sediment centrifugal with acetone and ether repeatedly washing skimmed over the coarse sugar papayin, except with more coarse sugar, protein, and the activated carbon used in pigment, and the phenol-sulfuric acid method measure sugar content

After gentrifugalism takes the precipitation to wash the degreasing repeatedly with the acetone ether, obtains the thick polysaccharide, with the papayin except thick polysaccharide in protein, the activated charcoal removing pigment, uses the phenol sulfuric acid method to determine its polysacchar

Take centrifugal precipitation using acetone diethyl ether after repeated washing-degreasing, be crude polysaccharide, in addition to coarse polysaccharide protein with papain, activated carbon for removal of pigment, determination of polysaccharide content by phenol-sulfuric acid method

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