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關注:1
2013-05-23 12:21
求翻譯:摘要:目的:用彗星實驗(comet assay)確定常規凍精的DNA損傷程度和加入抗壞血酸鹽后的精子損傷程度的方法:分別對奶牛的常規凍精以及加入抗壞血酸鹽后的凍精解凍、離心、洗滌,使用普通載玻片雙層鋪膠,在15V,150mA,4℃的條件下電泳45min,吖啶橙染色后在熒光顯微鏡下檢測、進行數據統計。結果:一定濃度的抗壞血酸鹽對精子具有保護作用。是什么意思?![]() ![]() 摘要:目的:用彗星實驗(comet assay)確定常規凍精的DNA損傷程度和加入抗壞血酸鹽后的精子損傷程度的方法:分別對奶牛的常規凍精以及加入抗壞血酸鹽后的凍精解凍、離心、洗滌,使用普通載玻片雙層鋪膠,在15V,150mA,4℃的條件下電泳45min,吖啶橙染色后在熒光顯微鏡下檢測、進行數據統計。結果:一定濃度的抗壞血酸鹽對精子具有保護作用。
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2013-05-23 12:21:38
Abstract: Objective: To determined using the comet assay (comet assay,) conventional frozen semen of dna damage and ascorbic acid salt, the degree of sperm damage: conventional frozen semen on dairy cattle and ascorbic acid salt, frozen semen thawed, centrifuged, washing, the use of ordinary slides
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2013-05-23 12:23:18
Summary: Purpose: The comet's lab (comet assay) to determine the general price level and join DNA damage ascorbic acid salt of the methods of sperm damage in cows, respectively: the conventional freeze-Boutique, as well as accession to ascorbic acid salt of a thaw, centrifugal, washing, and use a no
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2013-05-23 12:24:58
Abstract: Goal: After the comet experiment (comet assay) determined the conventional frozen fine DNA extent of damage and joins the antiscorbutic acid salt the sperm extent of damage method: Separately as well as joins the antiscorbutic acid salt af
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2013-05-23 12:26:38
Summary: purpose: with Comet experimental (Comet assay) determines General frozen fine of DNA injury degree and joined Ascorbic acid salt Hou of sperm injury degree of method: respectively on cow of General frozen fine and joined Ascorbic acid salt Hou of frozen fine thaw, and centrifugal, and washi
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2013-05-23 12:28:18
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