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關(guān)注：1
2013-05-23 12:21
求翻譯：(PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min. The tubes were incubated for 18 h, and the cell wall debris removed by centrifugation at 13,000 rpm. The amount of heme present in cleared cell lysate 是什么意思？ 待解決
 懸賞分：1
- 離問題結(jié)束還有
(PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min. The tubes were incubated for 18 h, and the cell wall debris removed by centrifugation at 13,000 rpm. The amount of heme present in cleared cell lysate
問題補(bǔ)充： |
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2013-05-23 12:21:38
（PBS），加上500升去纖維綿羊血（東北實(shí)驗(yàn)室服務(wù)，斯羅，我），細(xì)胞沉淀，離心4分鐘9000轉(zhuǎn)（6,605克）。管培養(yǎng)18小時(shí)，并在13000轉(zhuǎn)離心除去細(xì)胞壁碎片。在清除細(xì)胞裂解液中的血紅素由od415確定目前的數(shù)量作為衡量的紅血細(xì)胞裂解和歸到細(xì)菌細(xì)胞密度（OD600）。
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2013-05-23 12:23:18
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2013-05-23 12:24:58
(PBS)加上500 l defibrinated綿羊血液(東北實(shí)驗(yàn)室業(yè)務(wù)， Winslow，我)，并且細(xì)胞由離心法射擊在9,000轉(zhuǎn)每分鐘(6,605 g) 4 min。 管為18 h被孵化了，并且細(xì)胞壁殘骸由離心法去除了在13,000轉(zhuǎn)每分鐘。 相當(dāng)數(shù)量heme當(dāng)前在被清除的細(xì)胞lysate一樣堅(jiān)定由OD415使用了象紅血球病勢(shì)漸退措施并且正常化了到細(xì)菌細(xì)胞密度(OD600)。
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2013-05-23 12:26:38
(PBS) 加 500 l 菌種保存羊血 （東北實(shí)驗(yàn)室服務(wù)、 溫思勞，ME） 和單元格被顆粒用離心法在 4 分鐘的 9,000 rpm (6,605 g)。管子被孵化 18 h，和刪除用離心法在 13,000 rpm 的細(xì)胞壁碎片。目前已清除細(xì)胞是由 OD415 中血紅素的量是用作血紅細(xì)胞溶解的措施和規(guī)范化為細(xì)菌細(xì)胞密度 （OD600）。
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2013-05-23 12:28:18
(PBS) plus 500 l defibrinated sheep blood (Northeast Laboratories Services, Winslow, ME), and cells were pelleted by centrifugation at 9,000 rpm (6,605 g) for 4 min.管子為 18 h 被孵卵，以及在 13,000 rpm 處被離心分離撤銷的細(xì)胞墻碎片。在被弄清楚的細(xì)胞中的數(shù)量的 heme 禮物 lysate 如通過 OD415 決定用作紅血球消散的一種手段和到細(xì)菌的細(xì)胞密集正常化 ( OD600 )。
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