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關(guān)注:1
2013-05-23 12:21
求翻譯:The OD595 was determined for baseline optical density. The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well. The plates were allowed to dry, and then the cells were fixed at 80°C for 30 min. Following staining with 0.1% crystal violet for 20 min, the wells were 是什么意思?![]() ![]() The OD595 was determined for baseline optical density. The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well. The plates were allowed to dry, and then the cells were fixed at 80°C for 30 min. Following staining with 0.1% crystal violet for 20 min, the wells were
問(wèn)題補(bǔ)充: |
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2013-05-23 12:21:38
od595被確定為基準(zhǔn)的光密度。從板,這是蒸餾水200 l每洗兩次,然后被驅(qū)逐的文化。板被允許干,然后將細(xì)胞固定在80擄C的30分鐘。 20分鐘的0.1%結(jié)晶紫染色后,井用200升95%乙醇脫色。
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2013-05-23 12:23:18
正在翻譯,請(qǐng)等待...
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2013-05-23 12:24:58
OD595為基礎(chǔ)線光學(xué)密度是堅(jiān)定的。 文化從板材然后被逐出了,兩次洗滌與200 蒸餾水l每口井。 板材讓烘干,然后細(xì)胞是固定的在80°C 30 min。 跟隨的弄臟與0.1%結(jié)晶紫20分鐘,井是destained與200 l 95%對(duì)氨基苯甲酸二。
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2013-05-23 12:26:38
OD595 確定基準(zhǔn)光密度。然后,文化被驅(qū)逐出車牌的兩次與每井蒸餾水的 200 l 被沖走。盤(pán)子獲準(zhǔn)以干,然后單元格被固定在 80 ° C,30 分鐘。后 20 分鐘,0.1%結(jié)晶紫染色,井被 destained 200 l 95%乙醇。
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2013-05-23 12:28:18
OD595 為底線被確定光學(xué)密度。The cultures were then expelled from the plate, which was washed twice with 200 l of distilled water per well.盤(pán)子被允許變干,然后細(xì)胞持續(xù) 30 分鐘在 80 ° C 被固定。Following staining with 0.1% crystal violet for 20 min, the wells were destained with 200 l 95% ethanol.
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