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關注：1
2013-05-23 12:21
求翻譯：采用RT-PCR方法,利用牛病毒性腹瀉病毒(BVDV)C24V株接種MDBK細胞,提取病毒RNA，擴增出BVDV-E0基因,與pET28a原核表達載體分別經BamHⅠ和HandIII雙酶切,連接,經鑒定后證明成功構建pET28a-E0原核表達載體,經IPTG誘導通過SDS-PAGE和Western-blot檢測到目的蛋白表達,為研究蛋白功能和單克隆抗體的研制打下了基礎。是什么意思？ 待解決
 懸賞分：1
- 離問題結束還有
采用RT-PCR方法,利用牛病毒性腹瀉病毒(BVDV)C24V株接種MDBK細胞,提取病毒RNA，擴增出BVDV-E0基因,與pET28a原核表達載體分別經BamHⅠ和HandIII雙酶切,連接,經鑒定后證明成功構建pET28a-E0原核表達載體,經IPTG誘導通過SDS-PAGE和Western-blot檢測到目的蛋白表達,為研究蛋白功能和單克隆抗體的研制打下了基礎。
問題補充： |
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2013-05-23 12:21:38
By RT-PCR method, the use of bovine viral diarrhea virus (BVDV) C24V strains of MDBK cells inoculated, extracted viral RNA, BVDV-E0 gene was amplified with prokaryotic expression vector pET28a, respectively, by BamH Ⅰ and HandIII restriction enzyme digestion, ligation, by After identification proved
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2013-05-23 12:23:18
正在翻譯，請等待...
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2013-05-23 12:24:58
正在翻譯，請等待...
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2013-05-23 12:26:38
正在翻譯，請等待...
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2013-05-23 12:28:18
Adopt RT-PCR method, utilize the viral diarrhoea and virus (BVDV) C24V one of ox to inoculate MDBK cells, withdraws virus RNA, amplify out BVDV-E0 gene, express with pET28a originally and nuclearly carriers are cut by one pair of enzymes BamHⅠ and HandIII separately, connects, prove that succeeds in
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