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關注:1
2013-05-23 12:21
求翻譯:Western Blot采用的是聚丙烯酰胺凝膠電泳,被檢測物是蛋白質,“探針”是抗體,“顯色”用標記的二抗。經過PAGE分離的蛋白質樣品,轉移到固相載體(例如硝酸纖維素薄膜)上,固相載體以非共價鍵形式吸附蛋白質,且能保持電泳分離的多肽類型及其生物學活性不變。以固相載體上的蛋白質或多肽作為抗原,與對應的抗體起免疫反應,再與酶或同位素標記的第二抗體起反應,經過底物顯色或放射自顯影以檢測電泳分離的特異性目的基因表達的蛋白成分。該技術也廣泛應用于檢測蛋白水平的表達。是什么意思?![]() ![]() Western Blot采用的是聚丙烯酰胺凝膠電泳,被檢測物是蛋白質,“探針”是抗體,“顯色”用標記的二抗。經過PAGE分離的蛋白質樣品,轉移到固相載體(例如硝酸纖維素薄膜)上,固相載體以非共價鍵形式吸附蛋白質,且能保持電泳分離的多肽類型及其生物學活性不變。以固相載體上的蛋白質或多肽作為抗原,與對應的抗體起免疫反應,再與酶或同位素標記的第二抗體起反應,經過底物顯色或放射自顯影以檢測電泳分離的特異性目的基因表達的蛋白成分。該技術也廣泛應用于檢測蛋白水平的表達。
問題補充: |
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2013-05-23 12:21:38
正在翻譯,請等待...
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2013-05-23 12:23:18
Western Blot used the polyacrylamide gel electrophoresis, protein is detected, The property is "needle" antibodies, and "color" of markings used 2 anti-. As a result of separation PAGE protein samples, and transferred to the fixed-carrier (for example, cellulose nitrate film) on solid carriers to no
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2013-05-23 12:24:58
Western Blot uses is the polyacrylamide gel electrophoresis, by the test item is the protein, “the probe” is the immune body, “the coloration” uses the mark two anti-.After the PAGE separation protein sample, shifts to the solid phase carrier (e.g. nitrocellulose thin film) on, the solid phase carri
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2013-05-23 12:26:38
Western Blot using polyacrylamide gel electrophoresis, detection is a proteins, "probe" is the antibody, "colour" with Mark II anti-. After PAGE separation of protein samples transferred to the solid phase carrier (such as nitrocellulose film), the solid phase carrier proteins in the form of non-Cov
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2013-05-23 12:28:18
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