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fields: By setting up small district pilot, using 15 N natural abundance determination under different Shih nitrogen level of nitrogen-fixing Rhizobium strains vaccination efficacy and the alfalfa growth and seed production.

: Through establishes the field plot experiment, uses 15 N natural abundance law determination to execute under the nitrogen level to vaccinate differently the root nodule bacteria nitrogen fixation potency and to the alfalfa growth and the seed output influence.

: Community testing by setting the field, using the natural abundance of 15 n determination under different nitrogen application rates of nitrogen-fixing Rhizobium inoculation effectiveness and its impact on growth and seed yield of alfalfa.