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關(guān)注:1
2013-05-23 12:21
求翻譯:The tubes were incubated at 65°C for 15 min and were centrifuged again at 12000 rpm for 10 min. Equal volume of chloroform – isoamylalcohol (24:1) were added to the supernatants and the tubes were centrifuged at 12000 rpm for 10 min.是什么意思?![]() ![]() The tubes were incubated at 65°C for 15 min and were centrifuged again at 12000 rpm for 10 min. Equal volume of chloroform – isoamylalcohol (24:1) were added to the supernatants and the tubes were centrifuged at 12000 rpm for 10 min.
問題補充: |
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2013-05-23 12:21:38
管在65℃孵育15分鐘,再次離心10分鐘的轉(zhuǎn)速在12000。上清,分別加入等體積的氯仿 - 異戊醇(24:1),并在10分鐘的轉(zhuǎn)速12000離心管。
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2013-05-23 12:23:18
該管是培養(yǎng)皿置于65°C為15分鐘,在12000RPM centrifuged再次為10分鐘相同份量的三氯甲烷含量-isoamylalcohol(24:1),列入了supernatants和管是centrifuged在12000RPM10分鐘。
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2013-05-23 12:24:58
正在翻譯,請等待...
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2013-05-23 12:26:38
管孵出了 15 分鐘 65 ° C 和被再次離心為 12000 rpm 的氯仿 10 分鐘等于卷 — — isoamylalcohol (24: 1) 添加到上清并管有離心為 12000 rpm 10 分鐘。
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2013-05-23 12:28:18
管在65℃孵育15分鐘,再次離心10分鐘的轉(zhuǎn)速在12000。上清,分別加入等體積的氯仿 - 異戊醇(24:1),并在10分鐘的轉(zhuǎn)速12000離心管。
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