|
關(guān)注：1
2013-05-23 12:21
求翻譯：Single-cell reverse-transcription PCR (RTPCR). Single-cell RT-PCR was performed as previously described (Park et al., 2006; Liu et al., 2010). Briefly, following whole-cell patchclamp recordings, spinal lamina II neurons or DRG neurons were harvested into patch pipettes with tip diameters of 15–25 m, gently put into r是什么意思？ 待解決
 懸賞分：1
- 離問題結(jié)束還有
Single-cell reverse-transcription PCR (RTPCR). Single-cell RT-PCR was performed as previously described (Park et al., 2006; Liu et al., 2010). Briefly, following whole-cell patchclamp recordings, spinal lamina II neurons or DRG neurons were harvested into patch pipettes with tip diameters of 15–25 m, gently put into r
問題補(bǔ)充： |
|
2013-05-23 12:21:38
單細(xì)胞的反轉(zhuǎn)錄聚合酶鏈反應(yīng)（RT-PCR）。單細(xì)胞RT-PCR方法進(jìn)行，如前所述（公園等，2006;劉等人，2010）。簡(jiǎn)單地說，全細(xì)胞patchclamp錄音，脊髓Ⅱ板層神經(jīng)元和背根神經(jīng)節(jié)神經(jīng)元收獲到補(bǔ)丁移液器，尖直徑為15-25米，輕輕地放入含有反轉(zhuǎn)錄試劑的反應(yīng)管，在50℃保溫1 h（標(biāo)三，Invitrogen公司）。獨(dú)立的PCR的cDNA產(chǎn)品中使用了。使用的引物序列示于表1。進(jìn)行第一輪PCR在50 l的PCR緩沖液中含有0.2
|
|
2013-05-23 12:23:18
|
|
2013-05-23 12:24:58
|
|
2013-05-23 12:26:38
|
|
2013-05-23 12:28:18
|
湖北省互聯(lián)網(wǎng)違法和不良信息舉報(bào)平臺(tái) | 網(wǎng)上有害信息舉報(bào)專區(qū) | 電信詐騙舉報(bào)專區(qū) | 涉歷史虛無(wú)主義有害信息舉報(bào)專區(qū) | 涉企侵權(quán)舉報(bào)專區(qū)
