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關注:1
2013-05-23 12:21
求翻譯:The first step in blotting is the transfer of the nucleic acids from the gel to a membrane, which is conventionally done using the capillary transfer method. In this procedure, the gel is placed on top of a paper wick that dips into a reservoir containing the transfer buffer. The membrane is sandwiched between the gel 是什么意思?![]() ![]() The first step in blotting is the transfer of the nucleic acids from the gel to a membrane, which is conventionally done using the capillary transfer method. In this procedure, the gel is placed on top of a paper wick that dips into a reservoir containing the transfer buffer. The membrane is sandwiched between the gel
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2013-05-23 12:21:38
印跡法的第一步是從凝膠轉移的核酸的膜,這是以往使用毛細管轉移法完成。在此過程中,該凝膠被放置在頂部的紙燈芯浸入含有轉移緩沖器水庫。膜被夾之間的凝膠和一個堆棧的吸收材料(通常是干紙巾),用于繪制的轉移緩沖液通過凝膠通過毛細管作用。在凝膠中的核酸分子,然后進行由緩沖器成為固定于膜上。真空印跡是另一種雜交的過程,已越來越普及,因為它的速度更快,提供了良好的回收率。真空印跡還使用緩沖液的流傳輸到膜上的核酸的頻帶,但它使用了一個真空,而不是毛細管作用來創建流。將膜放置在多孔載體上,被放置在它的凝膠中,施加真空,緩沖區被添加到淹沒凝膠。
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2013-05-23 12:23:18
第一步是在赦免的轉讓從核酸的凝膠,一個膜,這是常規使用的毛細血管傳
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2013-05-23 12:24:58
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2013-05-23 12:26:38
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2013-05-23 12:28:18
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